Composite

Part:BBa_K5226086:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-30)


PRM-ci857-mmp1-fre-stth


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1973
    Illegal NgoMIV site found at 2231
    Illegal NgoMIV site found at 2934
    Illegal NgoMIV site found at 3200
    Illegal AgeI site found at 2119
    Illegal AgeI site found at 2460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1362
    Illegal SapI.rc site found at 1482


Design Notes

none


Source

fre is obtained through PCR amplification.Sequence source is from the genome of E.coli MG1655. stth is obtained through synthetic（BGI）. Sequence source is from Methylophaga aminisulfidivorans, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/AF494423)

References